Title:
Metronidazole nano polymers: Preparation, characterizations and release study / by Hazem Abdul Kader Sabbagh; supervised by Dr. Samer Hasan Hussein al-Ali, Dr. Zead Abudayeh. :
الميترونيدازول- نانوبوليمر التحضير، دراسة الصفات ودراسة التحرر
:
الميترونيدازول- نانوبوليمر التحضير، دراسة الصفات ودراسة التحرر :
الميترونيدازول- نانوبوليمر التحضير، دراسة الصفات ودراسة التحرر
Author:
Sabbagh, Hazem Abdul Kader, author.
al-Ali, Samer Hasan Hussein, supervisor.
Abudayeh, Zead, co-supervisor.
Isra University (Amman, Jordan). Faculty of Pharmacy. Department of Pharmaceutical Sciences.
General Notes:
Thesis (M.Sc in Pharmaceutical Science)-- Isra University (Amman, Jordan), Faculty of Pharmacy, Department of Pharmaceutical Sciences
, 2018.
Includes bibliographical references and index.
The administration of drugs designed to be given as a single dose rather than multiple doses has recently been made possible using extended release formulation approach; the release of the drugs can be accomplished over long periods of time, enabling an almost constant level of the drug to be maintained in the bloodstream. Moreover, extended release formulations increase the clinical efficacy of drugs. The introduction of drug nanocomposites as extended release vehicles has provided a breakthrough in novel drug delivery systems in the field of pharmaceutical technology, for which nanopolymers are widely used for this purpose. This study aimed to achieve the preparation of new extended release formulation of metronidazole (MET) via loading of the MET onto nanopolymers in order to increase the residence time in the body by extended release.
Three nanocomposites were prepared using chitosan (CSNPs), alginate (AlgNPs) and chitosan-alginate (CS-AlgNPs) nanoparticles; MET-CSNPs, MET-AlgNPs and MET-CS-AlgNPs nanocomposites, respectively. The prepared nanocomposites were studied for their physiochemical properties, loading efficiency (LE), encapsulation efficiency (EE), and release.
III
The MET-CSNPs nanocomposites were prepared by mixing different concentrations of CS (0.5, 1, and 2 mg/mL) with TPP solutions (0.5, 1, 2.5 mg/mL) under controlled pH at 4.5 using NaOH. The product was centrifuged at 10000 rpm for 15 min and dried. The same procedure was repeated for the preparation of MET-CSNPs nanocomposites using different concentrations of MET (100, 200, and 400 mg). The MET-AlgNPs were prepared by ionic gelation method between sodium alginate (1, 2 and 4 mg/mL) and cross-linking agent of CaCl2 (0.5, 0.75, and 1% w/v) in the presence of MET (100, 200, and 400 mg). MET-CS-AlgNPs nanocomposites were prepared using solutions of CS (0.5, 1 and 2 mg/mL), sodium alginate (2 and 4 mg/mL) and CaCl2 (0.5 and 1%) at 100 mg MET concentration. XRD spectra of MET-CSNPs showed two peaks at 2θ=12o and 23.7o, indicating amorphous forms due to cross-linkage with TPP. XRD spectra of MET-AlgNPs showed three diffraction peaks at 2θ values 14.1º, 21.8º and 39.1º. XRD patterns of MET-CS-AlgNPs showed overlap between patterns of CS and Alg, which lead to the appearance of peaks at 2θ = 14.5o and 20.6o. The FTIR data for all nanocomposites prepared in this work showed the spectra of nanopolymers (CSNPs, AlgNPs and CS-AlgNPs) as well as the spectra of MET. This result indicates the incorporation of MET in the nanopolymers.
Correlation and multiple regression analyses were used in this work to examine the relationship between dependant variables (EE, LE, particle size and zeta potential) and independent variables
IV
(concentrations of CS, TPP and MET) for all nanocomposites. At MET-CSNPs nanocomposites, the MET concentration had a significant effect on the EE, LE and particle size. The TPP concentration significantly affected the particle size and zeta potential. In addition, CS concentration only affected the LE and zeta potential. In the case of MET-AlgNPs nanocomposites, the MET concentration only had a significant effect on the zeta potential. In addition, the variable CaCl2 had a significant effect on the LE only. On the other hand, the Alg concentrations affected all dependant variables significantly except the zeta potential. In regards to the MET-CS-AlgNPs nanocomposites, the independent variables were Alg, CaCl2 and CS concentrations. The Alg concentrations significantly affected all dependant variables except EE. In addition, the CaCl2 concentrations significantly affected all dependant variables except LE, whereas the CS concentrations significantly affected the EE and particle size. Extended release study of MET from its respective nanocomposites was carried out using 0.1N HCl and showed that the release rate of MET from the MET-CSNPs nanocomposites was slower than MET-AlgNPs and MET-CS-AlgNPs. These results indicate extended release of the drug from its respective nanocomposites, and therefore these nanocomposites have good potential to be used as extended-release formulation of the drugs.
The electronic version is available in theses database \\ University of Jordan.
Includes abstracts in Arabic and English.
Subject:
Pharmacology
Metronidazole
DRUG -- DOSAGE FORMS.
Pharmaceutical technology.
Drug delivery systems
Drugs -- Controlled release
Dissertation Note:
Thesis (M.Sc in Pharmaceutical Science)-- Isra University (Amman, Jordan), Faculty of Pharmacy, Department of Pharmaceutical Sciences
, 2018.
Physical Description:
1CD-ROM : PDF.
Publication Date:
2018.
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